An economical adaptation of the RosetteSep™ procedure for NK cell enrichment from whole blood, and its use with liquid nitrogen stored peripheral blood mononuclear cells

Abstract

This study reports an economical adaptation of the RosetteSep™ procedure for enrichment of NK cells designed for whole blood and its use with liquid nitrogen stored peripheral blood mononuclear cells (PBMC). Combining 45×10 6 PBMC with 45×10 8 Alsevers' stored red blood cells (RBC) in 1 ml requires 50 μl of RosetteSep™ bifunctional antibody cocktail and provides NK cells of 99% purity with average yields of 43%.