An ecological understanding of urogenital microbiome and the relationship of urinary tract infection

Abstract

Objective To explore the relationship between female urinary tract host, environment, microbial flora and changes in the host's suitability, and to prove that the urinary tract has its characteristic flora.To investigate the relationship between bacterial flora changes and urinary tract infections, and to make conceptual changes in the diagnosis and treatment of urinary tract infections. Methods From February 2018 to March 2018, 16SrRNA gene sequencing was used to detect the urine samples of women of healthy postmenopausal group(n=5), healthy childbearing age group(n=5), and postmenopausal with urinary tract infection group(n=14), and the diversity and diversity of urogenital microbiome were analyzed. Results The female urine contains a wide variety of microbiome, and the microbiome constitutes a diversity of individual differences, generally one or two strains predominates. The dominant bacteria in the healthy childbearing age and postmenopausal group are mainly lactobacillus, gardnerella, and sphingomonas, while sphingomonas in the postmenopausal urinary tract infection group was abruptly decreased in abundance. In postmenopausal with urinary tract infections, the predominant microbiome was mainly enterobacteriaceae and lactobacillus, and the abundance of enterobacteriaceae was significantly higher than other two groups, while the abundance of lactobacilli was significantly higher in the healthy childbearing age group than in the postmenopausal period two groups. Conclusions The dominant urobacteria of the female urogenital are lactobacillus. The urinary microbiome of women with postmenopausal urinary tract infection shows a significant reduction in lactobacillus, sphingomonas, and an increase in the abundance of enterobacteriaceae, suggesting that this may be an important cause of the increased incidence of urinary tract infections in postmenopausal women. It provides ideas for diagnosing urinary tract infections and non-antibiotic means for the treatment of urinary tract infections. Key words: Urinary Tract Infections; Microbiota; Menopause; Fertility