An easy and effective method for radiolabelling of solid lipid nanoparticles

Abstract

Nanoparticle biodistribution study is of great importance in bringing nanomedicine to patients. In this article, solid lipid nanoparticle (SLN) with dimension less than 100 nm was successfully radiolabelled with 99mTc by using sodium borohydride as a reducing agent (instead of stannous salts). Paclitaxel (PTX) was used as a model anticancer drug for the preparation of drug loaded SLN (PSLN). PSLN was characterized by standard methods. Encapsulation efficiency for PTX in PSLN was estimated by HPLC. PTX (Taxol formulation) and PSLN were radiolabelled separately and subsequent characterizations of these complexes were performed. Greater than 95 % radiolabelling efficiency was achieved and the labelling efficiency was calculated to be more than 90 % upto 24 h. Both the above-mentioned complexes passed the in vitro stability tests. PSLN achieved more brain concentration than Taxol as determined by biodistribution studies. This type of radiolabelling technique can be useful in preclinical evaluation of drug loaded SLN.