An early role of maternal mRNA in establishing the dorsoventral pattern in pelle mutant Drosophila embryos

Abstract

Mutations of the maternal effect locus pelle (pll) cause dorsalized Drosophila embryos. In extreme mutants, the embryo develops into a long hollow tube of dorsal cuticular structures with no sign of ventral pattern elements. Injection of wild-type cytoplasm or poly(A) +RNA into mutant pll embryos partially restores the normal pattern. Rescuing activity is present in the wild-type cytoplasm until the late blastoderm stage, but is already absent from the poly(A) +RNA fraction by the time of pole cell formation. At the same time, pll embryos fail to respond to injected biologically active poly(A) +RNA. This indicates that pll + mRNA is lost early from the pool of maternal RNA and that there is a non-RNA component of rescue. This component, most likely the pll + protein, appears to be unequally distributed in wild-type embryos.