Abstract
A novel aza-coumarin-hemicyanine conjugated hybrid was prepared, which was found to be a promising colorimetric and ratiometric near-infrared (NIR) fluorescent probe for detection of bisulfite/sulfite in aqueous solution. This probe shows a rapid response (within 30s) and high selectivity and sensitivity for bisulfite/sulfite, giving distinct colorimetric and ratiometric fluorescence changes at 717 and 560nm. The detection limit for bisulfite was estimated to be 87nM. In addition, detection of bisulfite in food samples and bioimaging of both exogenous and endogenous bisulfite in living cells with this probe were successfully applied, which indicates that this probe holds great potential for bisulfite detection both in real samples and in living cells.
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