Abstract
Hamster small intestinal slices were incubated in small amounts of oleic acid-3H for 5 minutes, followed by incubations in buffer. After the initial incubation, about 75% of the tissue radioactivity was in triglyceride with lesser amounts in phospholipid and other neutral lipids. During the second incubation in buffer, the radioactivity within the triglyceride fraction descreased to about 50% of the total tissue radioactivity due to a loss of triglyceride from the tissue. As a result of the triglyceride loss, the labeled phospholipid fraction now made up about 20% of the total tissue radioactivity. Radioactive analysis indicated a lipid loss of 16% during preparation of OsO4-fixed samples of this tissue for electron microscopy. In good agreement, chemical analysis indicated that 18% of the phospholipid and 21% of the neutral lipid was lost during preparation for electron microscopy. Electron microscopic autoradiographic analysis showed that after incubations for 10 or 20 minutes, grains were localized to the Golgi zones of the intestinal epithelial cells.
Published Version
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