An automated flow-injection serial dynamic dialysis technique for drug-protein binding studies

Abstract

An automated flow-injection analyzer is interfaced with a dialysis unit to study drug-protein protein binding interactions. The binding of some sulfonamides to bovine serum albumin is studied by means of the automated system and gives results similar to those obtained by other procedures. A usual time for a complete run is 100 min, including calibration. The dialysable sulfonamides are quantified spectrophotometrically by using a modified Bratton-Marshall method. The system is also used for the calculation of dialysis rate constants.