Abstract
Fish otoliths are generally composed of aragonite crystals. A large number of trace elements such as Sr, Fe, Mn, and Zn are included in the crystal. Especially, Sr enters the crystals much among those metals in the process of precipitation of the calcium carbonate. In addition, it is expected that Sr/Ca concentration ratios can indicate ambient temperature. In-air PIXE was adopted to analyze trace elements in otoliths of juvenile red sea bream. Preliminary results indicated that the Sr/Ca ratios of the otoliths increased proportional to rearing temperature and that PIXE was a fast and convenient technique for determination of several elements in fish otoliths.
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