An Astragalus membranaceus based eco-friendly biomimetic synthesis approach of ZnO nanoflowers with an excellent antibacterial, antioxidant and electrochemical sensing effect.

Abstract

Nowadays featuring outstanding eco-friendliness, the phytochemical fabrication method of nanostructures is very popular. Here, we propose to utilize the Astragalus membranaceus extract as the reducing and capping agent to stabilize the metal and to avoid the aggregations of nanoparticles during ZnO nanoflowers synthesis procedure. As a result, the whole fabrication procedure was highly efficient and cost-effective without requiring a special environment of high pressure or elevated temperature and without chemical hazards used or produced. After the fabrication, detailed characterization about material morphology and crystal structure was carried out, including scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscope (FTIR). Moreover, the ZnO nanoflowers demonstrated distinctive antibacterial, antioxidant and electrochemical sensing effect. Specifically, ZnO nanoflowers had an antibacterial inhibition zone of 19(±0.7) and 15(±0.8) mm in diameter against the concentration of 50μL (1mg/mL) Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), which is greatly improved compared to the reference drug (Kanamycin). Besides, antioxidant activity was also tested using H2O2 free radical scavenging assay and 60% 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition of 0.5mg/mL was reported. Finally, controlled by the diffusion process during the charge transfer procedure, 4-nitorphenol was dramatically reduced and a limit of detection of 0.08μM by ZnO nanoflowers modified electrode was observed during the cyclic voltammetry (CV) experiment. Because the phenolic compounds originating from Astragalus membranaceus helped to facilitate the electron transfer, the limit of detection was lower compared to other materials, such as copper oxide nanoparticles (Cu2O-NPs), silicon dioxide/silver nanoparticles (SiO2/Ag-NPs), zinc oxide nanoparticles (ZnO-NPs), activated carbon (AC) and cobalt oxide nanocubes (Co3O4). Therefore, featuring easy operation, low-cost and eco-friendliness, our proposed ZnO nanoflowers fabrication method will have a great potential in biomedical and electro-catalytic fields.