Abstract
Supercritical fluid chromatography (SFC) has seen a recent resurgence in interest following investment in the development of instrument technology by numerous instrument manufacturers. Increased focus on sustainability in chromatographic science, coupled with the orthogonality to reversed phase HPLC, is likely to further drive the uptake of SFC in many sectors. As with any form of chromatography, optimizing separation selectivity is a key variable in providing adequate resolution and accurate identification and quantification of target analytes. Stationary phase chemistry can be readily exploited to substantially alter the separation selectivity obtained. This article examines and characterizes the selectivity differences offered by three prototype SFC phases.
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