An assessment of chromic oxide as an indigestible marker for digestion studies in sheep

Abstract

SUMMARYFistulated cross-bred sheep were given, by means of continuous feeders, concentrate diets containing chromic oxide (Cr2O3). Dry-matter digestibility coefficients predicted from the faecal concentration of Cr2O3 were compared with those obtained by total collection of faeces, and the concentration of Cr2O3 in spot samples of faeces taken at different times of day was compared with that in faeces bulked over an 8-day collection period. The rate of flow of digesta from the abomasum and the partition of digestion between the stomach and intestines, calculated from the concentration of Cr2O3 in samples of abomasal digesta, were compared with values obtained using two markers, one of which remained in solution while the other was adsorbed by particulate matter.Récovery of Cr2O3 in the faeces ranged from 91 to 101% and predicted dry-matter digestibilities were similar to those obtained by total collection. There was no diurnal variation but there was marked random variation in the concentration of Cr2O3 in spot samples of faeces. Cr2O3 was shown to behave independently of the particulate matter in digesta and, as a single marker, it significantly underestimated the flow of digesta from the abomasum relative to the double marker method. Cr2O3 thus overestimated digestion in the stomach and underestimated digestion in the intestines. It was concluded that Cr2O3 was not a satisfactory marker for studies of the flow of digesta and hence the partition of digestion within the gastro-intestinal tract when digesta samples are taken from a simple cannula.