Abstract
BackgroundSeveral mosquito collection methods are routinely used in vector control programmes. However, they target different behaviours causing bias in estimation of species diversity and abundance. Given the paucity of mosquito trap data in West Africa, we compared the performance of five trap-lure combinations and Human Landing Catches (HLCs) in Guinea.MethodsCDC light traps (LT), BG sentinel 2 traps (BG2T), gravid traps (GT) and Stealth traps (ST) were compared in a 5 × 5 Latin Square design in three villages in Guinea between June and July 2018. The ST, a portable trap which performs similarly to a LT but incorporates LEDs and incandescent light, was included since it has not been widely tested. BG2T were used with BG and MB5 lures instead of CO2 to test the efficacy of these attractants. HLCs were performed for 5 nights, but not as part of the Latin Square. A Generalised Linear Mixed Model was applied to compare the effect of the traps, sites and collection times on mosquito abundance. Species identification was confirmed using PCR-based analysis and Sanger sequencing.ResultsA total of 10,610 mosquitoes were captured across five traps. ST collected significantly more mosquitoes (7096) than the rest of the traps, but resulted in a higher number of damaged specimens. ST and BG2T collected the highest numbers of Anopheles gambiae (s.l.) and Aedes aegypti mosquitoes, respectively. HLCs captured predominantly An. coluzzii (41%) and hybrids of An. gambiae and An. coluzzii (36%) in contrast to the five traps, which captured predominantly An. melas (83%). The rural site (Senguelen) presented the highest abundance of mosquitoes and overall diversity in comparison with Fandie (semi-rural) and Maferinyah Centre I (semi-urban). Our results confirm the presence of four species for the first time in Guinea.ConclusionsST collected the highest number of mosquitoes suggesting this trap may play an important role for mosquito surveillance in Guinea and similar sites in West Africa. We recommend the incorporation of molecular tools in entomological studies since they have helped to identify 25 mosquito species in this area.
Highlights
Several mosquito collection methods are routinely used in vector control programmes
Stealth traps (ST) collected the highest number of mosquitoes suggesting this trap may play an important role for mosquito surveillance in Guinea and similar sites in West Africa
The ST captured the highest percentage of the total number of mosquitoes collected (67%), followed by the light traps (LT) (24%), the BG2T with MB5 lure (BG2-MB5) lure (4%), the gravid traps (GT) (3%) and the BG2T with BG lure (BG2-BG) lure (2%) (Table 1)
Summary
Several mosquito collection methods are routinely used in vector control programmes. They target different behaviours causing bias in estimation of species diversity and abundance. Control programmes which target malaria and other vector-borne diseases need to be specific to the country or region in which they are implemented. In order to choose the best intervention(s), it is essential to know which mosquito species are both present and transmitting human pathogens in a given area. Despite primary vectors contributing to the majority of the transmission of mosquito-borne diseases, secondary vector species can play an essential role in maintaining residual transmission [1], be less affected by interventions focused on primary vectors and increase in dominance and relative importance [2, 3]. In order to monitor the effectiveness of a control programme, mosquito abundance and composition before and after intervention deployment can be determined by undertaking entomological surveys
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
