Abstract
IEF of humic and fulvic acids (HA and FA) from various sources were carried out on a flat-bed of Sephadex gels G-75 with Ampholine of appropriate pH-range. From the Sephadex gels, fractions of a FA sample were collected separately. It was found that each FA fraction of a certain pH value will precipitate out in Ampholine solution of higher pH.Infrared spectrumof the precipitate identified the formation of a complex between FA and Ampholine. The gas chromatograms of two adjacent methylated FA fractions showed compounds in the two fractions were mostly identical, but their relative amount differed somewhat. From these results, we may conclude that IEF is a usable technique for the characterization of humic substances rather than as a separation technique.
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