An aqueous mediated ultrasensitive facile probe incorporated with acrylate moiety to monitor cysteine in food samples and live cells

Abstract

A colorimetric probe TQA ((E)-4-(((8-(sec-butoxy)-2,3,6,7-tetrahydro-1H,5H-pyrido[3,2,1-ij]quinolin-9-yl)methylene)amino)benzylacrylate) possessing greater potent towards the sensing of cysteine was successfully synthesized and characterized. The aqueous soluble probe TQA detects Cys based on “ON-OFF” effect with excellent absorbance and emission properties. The probe TQA detects Cys up to its ultra-low level concentration of 1.5 nM and also quantifies the Cys up to 5.05 nM with the quicker response time of 140 s (2.3 min). In addition, the color change produced by the probe TQA on integrated with Cys was also identified easily via paper strip, cotton wool buds and RGB color picker app in smart mobiles. Further, the admirable selectivity and sensitivity of the probe TQA towards Cys extends its utility towards food samples and imaging of live HeLa cells.