Abstract
Improvement of methods for reliable and early diagnosis of the cellular diseases is necessary. A biological selectivity probe, such as an aptamer, is one of the candidate recognition layers that can be used to detect important biomolecules. Lung cancer is currently a typical cause of cancer-related deaths. In this work, an electrical sensing platform is built based on amine-terminated aptamer modified-gold electrodes for the specific, label-free detection of a human lung carcinoma cell line (A549). The microdevice, that includes a coplanar electrodes configuration and a simple microfluidic channel on a glass substrate, is fabricated using standard photolithography and cast molding techniques. A procedure of self-assembly onto the gold surface is proposed. Optical microscope observations and electrical impedance spectroscopy measurements confirm that the fabricated microchip can specifically and effectively identify A549 cells. In the experiments, the capacitance element that is dominant in the change of the impedance is calculated at the appropriate frequency for evaluation of the sensitivity of the biosensor. Therefore, a simple, inexpensive, biocompatible, and selective biosensor that has the potential to detect early-stage lung cancer would be developed.
Highlights
During the past three decades, many diseases in humans have emerged strongly, including cancer.Lung cancer is one of the most frequently-recognized cancers in both men and women, with over1.5 million new cases occurring per year, accounting for about 13% of total cancer diagnoses [1].The existing diagnosis methods, which are based on histological examinations of the suspicious tissue in the context of its clinical and morphological features [2], are often very expensive, and require advanced instruments
In the current we compared the specificity among these human adenocarcinoma cells from various tissues including study, we compared the specificity among these human adenocarcinoma cells from various tissues lung, cervix, stomach, and colon: A549, Hela (human cervical including lung, cervix, stomach, and colon: A549, Hela cancer cells), MKN45 and Caco-2
A549 cells were chosen as the target cells, whereas Hela cells, MKN45 cells, Caco-2 cells, cells (RBCs collected from volunteers) were the non-target cells used to evaluate the affinity and and red blood cells (RBCs collected from volunteers) were the non-target cells used to evaluate the selectivity of the sensing probes
Summary
The existing diagnosis methods, which are based on histological examinations of the suspicious tissue in the context of its clinical and morphological features [2], are often very expensive, and require advanced instruments. They are not sensitive enough to diagnose the disease in its early stages, and are non-specific for cancer classification. Cancer cells can be found in many different states due to differences at the morphological and molecular levels [3,4]. Developing sensitive and specific approaches for the detection of cancerous cells is crucial.
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