An approach to toxicity of Fusarium mycotoxin, DON: Use in co-culture system with human intestinal cells.

Abstract

We introduce an approach to measuring the toxicity of mycotoxins using co-culture systems with human intestinal cells and target calls. The system is a simple method of interfacing Using chambers systems with commercially available filter supports on which epithelial monolayers can be grown. Bing cultured target calls of mycotoxin, such as lymphocyte, neuro cells, hepatocyte etc., can be cultured under the grown epithelial monolayer cells, and the responses of target cells are examined when the epitheilal cell was exposed to contaminants on the apical side of intestinal epithelial cells. As an application of this system, we examined the toxicity of deoxynivalenol (DON) on limphocytes through intestinal epithelial cells, Caco-2 cells. We found here that the exposure of intestinal epithelial cells to DON in production of cytokines (IL-10 and IL-12) on lymphocytes.