An approach to solventless sample preparation procedure for pesticides analysis using solid phase microextraction/supercritical fluid extraction technique

Abstract

Solid phase microextraction (SPME)/supercritical fluid extraction (SFE) technique was investigated to realize the approach to the true solventless sample preparation procedures for pesticides analysis. Pesticides in spiked water samples were concentrated by SPME technique and were desorbed from the SPME fiber coating with supercritical carbon dioxide as the desorption solvent, prior to their analyses with high performance liquid chromatography (HPLC). Preliminary experiments were performed in order to optimize the desorption conditions of pesticides from the SPME fiber coating using supercritical carbon dioxide as the desorption medium. Subsequently, extraction conditions of pesticides from spiked water samples with SPME technique were optimized using the optimized desorption conditions. Limit of detection of some pesticides used in this study was lower than the Japanese regulatory limits for environmental water in a golf course. Linearity was found between the tested concentration range between 0.1 and 5.0 mg l −1 with the R.S.D. between 7.4 and 12.0%.