Abstract
Staphylococcal enterotoxin A (SEA) is an extremely heat stable, highly toxic, and potent gastrointestinal toxin responsible for Staphylococcal intoxication in humans. Therefore, production of immunological reagents used for SEA detection is of prime importance. For this, two fusion expression vectors, one with pelB leader, the other without it were constructed and then transformed into E. coli BL21(DE3) to produce the single chain variable antibodies (ScFv) against SEA. Sodium dodecyl- sulphate-polyacrylamide gel electrophoresis, western blot and enzyme-linked immuno-sorbent assay were used for characterization of the ScFv. Results demonstrate that expression level of the soluble ScFv with pelB leader was 20 times higher than that without it and the binding activity of ScFv without pelB leader was far below as compared to the ScFv with it. This work provides an alternative method of fast and cheap production of soluble ScFv against SEA.
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