Abstract
A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and Brillita. Plant cultures were developed in a controlled environment with 0.4 MPa CO2 enrichment, sucrose-reduced medium, and light intensity of 60 mM m-2·s-1. Principal component analysis showed that component 1 (C1) grouped 64.08% of the total variability, while the first two components accounted for 86.97%. Representation of the principal components demonstrated three clusters corresponding with the blueberry genotypes, and within each cluster plants micropropagated in agar-base medium grouped separately from those plants multiplied in TIBs. Both plant number and total internodes traits (related to the productive efficiency) were demonstrated superior in blueberries propagated in TIBs. Additionally, when transferred to greenhouse conditions, blueberries propagated in TIBs showed higher adaptability and growing rates than those cultured by the conventional approach, altogether evidencing the occurrence of a photomixotrophic stage in the vitroplantlets cultured in TIBs.
Highlights
The blueberry (Vaccinium corymbosum L.) a tetraploid perennial, rhizomatous, cross-pollinated shrub [1], is a commercially important crop worldwide, and Chile is one of the main producers and exporting countries [2]
A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and Brillita
Principal component analysis showed that component 1 (C1) grouped 64.08% of the total variability, while the first two components accounted for 86.97%
Summary
The blueberry (Vaccinium corymbosum L.) a tetraploid perennial, rhizomatous, cross-pollinated shrub [1], is a commercially important crop worldwide, and Chile is one of the main producers and exporting countries [2]. Conventional micropropagation of V. angustifolium based in gelled medium has been established and reported [8,9,10,11]. In the case of lowbush blueberry (V. angustifolium Ait.), a protocol using RITA® bioreactors combined with semisolid gelled medium has been developed for the cultivar Fundy and for two wild clones [18]. Because the high production costs render conventional micropropagation systems (agar-based) less suitable for large-scale production, liquid cultures and automation has the potential to resolve the manual handling of the various stages of micropropagation [15]. Bioreactor cultures permit an additional management of both physical and chemical environments, such as air exchange, photosynthetic photon flux, and CO2 content, resulting in more efficient plant performance during the in vitro micropropagation [19,20,21]
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