An appraisal of antimicrobial, cytotoxic and phytotoxic potential of Oligomeris linifolia

Abstract

The aim of study was to evaluate the antimicrobial, cytotoxic and phytotoxic activities of the Oligomeris linifolia. The organic extracts were obtained by extraction the whole dried palnt with dichloromethane and methanol. The antibacterial activity was determined by inhibitory effect on the growth of Eschericha coli, Bacillus subtilis, Shigella flexinari, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi by using Agar tube diffusion method. The antifungal potential of the extracts was determined against growth of Candida albicans, Aspergillus flavus, Microsporum canis, Fusarium solani, Candida glabrata .Cytotoxic activity was evaluated by brine shrimp lethality test and phytotoxic activity was conducted against growth of Lemna minor frond. The phytochemical screening led to the presence of cardiotonic glycosides, anthraquinones and saponins in the plant. Methanol extract exhibited substantial antibacterial activity against Pseudomonas aeruginosa. In antifungal bioassay, Methanol extract showed 50% inhibition against Microsporum canis, while dichloromethane extract showed 40% inhibition against Aspergillus flavus and Fusarium solani as compared to standard drug. The dichloromethane extract showed significant activity at highest dose while the methanol extract showed good activity at highest dose against Lemna minor in phytotoxic bioassay. In Brine shrimp lethality bioassay methanol extract showed significant cytotoxicity at highest level of dose with LD50 462.40.The study concluded that Oligomeris linifolia has noteworthy antimicrobial, phytotoxic and cytotoxic potential.