An application of Iatroscan thin-layer chromatography with flame ionization detection—lipid classes of microorganisms as biomarkers in the marine environment

Abstract

Iatroscan TLC-FID combines the separatory capabilities of thin-layer chromatography (TLC) (on silica coated chromarods) with the possibility of quantitation by flame ionization detection (FID). It can be used to separate lipid extracts into classes of compounds ranging from neutral to polar products. The rapidity with which this system provides lipid class profiles from small samples makes it particularly useful in oceanographic studies where routine analyses of large numbers of samples are required. Lipid classes were analysed in 4 marine bacterial strains grown on various substrates and 10 microalgae isolated from the marine environment and grown in batch cultures. Data on lipid composition of these microorganisms were obtained and compared to literature data. Results show that ratios between classes of compounds within the different groups of microorganisms were characteristic enough to constitute a good print of bacterial or phytoplankton sources in organic matter in sea water. In a preliminary study, phospholipid classes from a bacterial strain and a microalga were separated. Results show that the determination of ratios between phospholipid classes in organic matter could represent an improvement in the utilization of lipid classes as biomarkers.