Abstract
A microscope fitted for phase contrast observation is modified so that a biological specimen in the centre of the field of view may be irradiated with α-particles. A beam of α-particles is obtained from a polonium source by a glass capillary collimating tube of 15 μ internal diameter, the source and collimator being mounted in a tube passing axially through the optical condenser. The irradiation rate is of the order of 1 particle/min and 70% of the particles lie within a circle of 30 μ diameter in the focal plane of the objective. A statistical method is used to calculate the probability of irradiation of a target of known size which is irradiated for a known time.
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