An apparatus for thin layer vertical polyacrylamide gel electrophoresis with discontinuous buffer and gel system

Abstract

A vertical polyacrylamide gel slab electrophoresis apparatus with a discontinuous gel and buffer system and a running gel of 1 mm in thickness was devised. Using this apparatus, which employs stacking and sieving effects, sharp bands comparable to those of disc electrophoresis were obtained. Furthermore, a new application using detection with ultraviolet method was introduced for isozyme study.