Abstract
Since freezing live cell suspensions has been standardized, the equally important thawing operation needed some degree of control. An apparatus is described that permits living cells to be thawed under controlled conditions with results that can be reproduced. Rapid thawing of frozen ampules containing live cell suspensions was previously accomplished in this laboratory by shaking the ampules manually in a 37°C water bath. The development of the thaw-bath apparatus described has made possible the revision of this thawing method. In addition, the apparatus protects the operator from glass fragments that may result if an ampule ruptures under high internal pressures.
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