An apigenin-induced decrease in K-antigen production by Sinorhizobium sp. NGR234 is y4gM- and nodD1-dependent

Abstract

Cultured cells of Sinorhizobium sp. NGR234 produce an abundance of capsular polysaccharides, or K antigens; however, cells that are cultured in the presence of apigenin, a nod gene inducer, exhibited a significant reduction in K-antigen production. The flavonoid-induced modulation in capsule production appeared to be related to the phase-shift changes associated with bacteroid differentiation. Therefore, the polysaccharides were extracted from Sinorhizobium sp. NGR234 bacteroids recovered from Vigna unguiculata cv Red Caloona root nodules, and subsequent analyses showed that the bacteroid extracts were virtually devoid of K-antigen. Polysaccharide extracts from two nodulation mutants cultured in the presence of apigenin were then analyzed, and the results showed that the flavonoid-inducible decrease in K-antigen production is y4gM- and nodD1-dependent.