Abstract
A strain of Musca domestica L. isolated from an insecticide-resistant strain was selected for 35 generations with increasing concentrations of apholate on sugar bait. Selection was begun with 0.05 and 0.1% apholate fed as sugar bait along with reconstituted skim milk. Resistance to apholate was apparent in the 15th generation after initiation of apholate-selection pressure. The concentration of apholate in sugar bait fed to flies was increased 26-fold in the 35 generations. Hatchability tests showed about a 5-fold increase in resistance compared with the parent insecticide-resistant strain. The strain showed a low level of cross resistance to metepa but not to methotrexate. After 35 generations of selection with apholate the strain showed a decrease in resistance to lindane, diazinon, dimethoate, and dimetilan. It could not be determined whether this regression of resistance was due to apholate selection or to cessation of insecticide-selection pressure.
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