Abstract
Present research work represents antiviral and antibacterial value of body fat of Saara hardwickii commonly called as spiny tailed lizard. Oil was extracted from body fats located in the ventral region of this animal using hydrocarbons e.g., n-hexane, methanol, butanol and ethyl acetate as a solvent. The antibacterial activity of lizard oil was tested against standard as well as multi-resistant lines ofEscherichia coli, Styphalococcus aureus, Pseudomonas aeruginosa and Proteus vulgaris alone and with antibiotic ampicillin. For antibacterial potential, Ethyl acetate and Butanol solvent extract showed best zone of inhibition (7mm) with P. aeruginosa and S. aureus respectively. For antiviral potential, Butanol and Methanol extract showed best HA (Hemagglutination) titer of 04 with NDV and IBV viral strain respectively. It is concluded that lizard oil has antimicrobial potential against different pathogens strains (virus, bacteria).
Highlights
The eastern spiny tailed lizard Saara hardwickii is found in Pakistan, India, and Afghanistan
Animal was captured from Cholistan Desert of Punjab, Pakistan, and four different extracts of lizard’s oil were prepared by using the solvents; n-hexane, butanol, ethyl acetate and methanol as described by (Dias et al, 2013).The oil was mixed with the four solvents to make the perfect extract named as n-hexane, butanol, ethyl acetate and methanol
Antiviral activity against Newcastle Disease Virus (NDV): The antiviral potential of oil extracts prepared were tested against NDV virus was determined in term of hemagglutination test (HA)
Summary
The eastern spiny tailed lizard Saara hardwickii is found in Pakistan, India, and Afghanistan. Saara hardwickii has a dorso ventrally bedfast physique with blubbery spiny tailed whorls of annoying scales (Hashmi and Khan, 2014). The first traditional medicine made from spiny tailed lizard’s parts and products in early 1990s was used for treatment of 20 critical illnesses such as diabetes, heart diseases, hypertension, gout, kidney problems and sexual dysfunction (Yuan et al, 2016). The lizards named Varanus niloticus, Varanus bengalensis body parts have been used to cure the diseases in China, Sudan and India. They have multiple medicinal uses and can be employed to treat more than one ailment.
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