Abstract
The pineapple stem juice-fraction was found to exhibit inhibitory effect against hemolytic activity of venom (hemolysin) prepared from the Deadly Box Jellyfish (Sea Wasp, Habu-Kurage), Chiropsalmus guadrigatus. The antihemolysin was purified to homogeneity from pineapple stem juice by Sephadex G-25 column chromatography, TLC, and HPLC using a MiniQ PC32 2/3 column. The purified antihemolysin had the maximum absorption value at 260nm. It was peptide containing fourteen kinds of amino acids. By the addition of 100μg/ml of antihemolysin, a half of original hemolytic activity of hemolysin was lost.
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