Abstract

At the present time animal cell culture is more significant and multifarious application tool for current research streams. A lot of field assorted from animal cell culture such: stem cell biology, IVF technology, cancer cell biology, monoclonal antibody production, recombinant protein production, gene therapy, vaccine manufacturing, novel drug selection and improvement. In this review conclude animal cell culture as well as its requirements.

Highlights

  • Tissue Culture is a general idiom used for the removal of cells, tissues, or organs from an animal and their placement into an artificial environment conductive to growth

  • A lot of field assorted from animal cell culture such: stem cell biology, IVF technology, cancer cell biology, monoclonal antibody production, recombinant protein production, gene therapy, vaccine manufacturing, novel drug selection and improvement

  • At present remarkable association in the field of animal cell culture done by researchers: Human insulin became the earliest recombinant protein to be approved as a therapeutic agent, Human growth hormone produced from recombinant bacteria was established in favor of beneficial use, plasminogen activator early recombinant animal cells became commercially accessible, [1]

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Summary

INTRODUCTION

Tissue Culture is a general idiom used for the removal of cells, tissues, or organs from an animal and their placement into an artificial environment conductive to growth. Tissue culture is capable of clear as the growth of tissue or cell separate from the organism. It is known as techniques of keeping tissues alive and growing in an appropriate culture medium. Growing tissues of living organism outside the body is made possible in an appropriate culture medium, containing mixture of nutrient either in solid or liquid form. 1897 Demonstrated the survival of cells isolated from blood and connective tissue in serum and plasma. 1949 Polio virus could be grown on human embryonic cells in culture 1952 Continuous cell line from a human cervical carcinoma known as hela (helen lane) cells 1955 Nutrient requirements 1964 Hat medium for cell selection

Laminar Flow Hoods
CO2 Incubators
Microscopes
Centrifuges
Freeze
SUBSTRATES IN FAVOR OF CELL DEVELOPMENT
MEDIA REQUIREMENT FOR CELL CULTURE
Basic Components in the Culture Media
CULTURE ENVIRONMENTS
TYPES OF CELLS
PROCEDURE OF CELL CULTURE
Type of Cell Culture
CELL LINE
Continuous Cell Lines
PRESERVATION AND STORAGE
10. CELL CULTURE POSSIBLE PROBLEMS
10.2. Bacteria and Fungi Contamination
10.3. Mycoplasma Contamination
10.4. Chemical Contamination
10.5. Antibiotics
11. CONCLUSION
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