An Anatomical Study of Rhizome Bud Formation Induced by Paclobutrazol and Adventitious Root Formation in In Vitro Cultures of Lapageria rosea (Ruiz et Pav.)

Abstract

An anatomical study was made of bud dimorphism in in vitro shoot cultures of Lapageria rosea cv. Nashcourt, utilizing the presence and absence of the gibberellin-biosynthesis inhibitor paclobutrazol (10 μM) in the medium to control the development of axillary buds. Patterns of axillary bud development differed between the aerial pattern of shoot extension (in the absence of paclobutrazol) and rhizome bud formation (in the presence of paclobutrazol), with respect to planes of cell division, cell expansion and the formation of adventitious root primordia. These differences are examined and discussed.