Abstract
There has been numerous attempts to link the ATP content of activated sludge to certain wastewater characteristics or to certain control parameters used in the operation of wastewater treatment plants. Published results vary and result from the use of different ATP extraction and measurement methods. This study deals with the development of an analytical method that requires filtration on polycarbonate membrane (0.4 μm) and a subsequent ATP extraction with DMSO. The filtration step overcomes problems of enzymatic inhibition in the firefly bioluminescence assay due to dissolved substances in the liquid fraction of the activated sludge samples. In comparison with extraction in boiling TRIS buffer, the most widely used method, extraction in DMSO was found to be effective and advantageous owing to its simplicity and reliability, the ease of its execution, and its potential use in routine measurements. The application of this analytical procedure to aerobic sludges in batch culture yielded a higher estimate of the average value of the ATP pool than previously published results.
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