Abstract
ABSTRACT: A high‐performance liquid chromatography/evaporative light scattering detection (HPLC/ELSD) method was developed to determine natural forms of soyasaponin B with (αg, βg, βa, γg, γya) and without (V, I, II, III, IV) 2,3‐dihydro‐2,5‐dihydroxy‐6‐methyl‐4H‐pyran‐4‐one (DDMP) in soybean. The coefficient of variance of intra‐day assays was < 9.51% and that of inter‐day assays was < 10.91%. The soyasaponin I was used as external standard for quantification of all soyasaponins because it was established that their ELSD signal responses were essentially the same under the specific operating conditions. The extraction conditions were optimized and the extract storage conditions were established for analysis of soyasaponin Bs in their natural state. The energy of activation of soyasaponin Pg was 40.76 kJ/mol. The recovery rates for soyasaponin I and II were 98.3% and 93.1%, respectively. The average of total soyasaponins content from 5 soybean samples was 3.37 μmol/g with ranges from 2.78 to 4.03 μmol/g. This method is simple and easy to use, and the sample preparation takes less time than existing methods.
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