Abstract

Uric acid is an important indicator of the insect infestation assessment in flour. In this work, we propose a method for uric acid detection based on voltammetry. This technique is particularly considered for the physicochemical properties of flour and contains a simple pretreatment process to rapidly achieve extraction and adsorption of uric acid in flour. To achieve specific recognition of uric acid, graphene and poly(3,4-ethylenedioxythiophene) (PEDOT) were used for the adsorption and concentration of uric acid in flour. The adsorbed mixture was immobilized on the surface of a screen-printed electrode for highly sensitive detection of the uric acid. The results showed that electrocatalytic oxidation of uric acid could be achieved after adsorption by graphene and PEDOT. This electrocatalytic reaction allows its oxidation peak to be distinguished from those of other substances that commonly possess electrochemical activity. This voltammetry-based detection method is a portable and disposable analytical method. Because it is simple to operate, requires no professional training, and is inexpensive, it is a field analysis method that can be promoted.

Highlights

  • Wheat is one of the most important food crops, accounting for 22% of total grain production

  • Preparation of Graphene/PEDOT Composite The graphene/PEDOT composite was synthesized by a microwave based method [28]

  • Electrochemical Sensor Fabrication and Measurement The synthesized graphene/PEDOT composite was first dispersed into ethanol to form a 0.1 mg/mL solution

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Summary

Introduction

Wheat is one of the most important food crops, accounting for 22% of total grain production. The electrochemical detection of uric acid has been extensively studied, mainly for medical purposes [25,26,27]. These electrochemical sensors provide highly sensitive detection of uric acid. These sensors are designed primarily for the biological environment of the human body and cannot be used directly for the detection of uric acid in flour.

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