An Analysis of the Yeast Flora Associated with Cactiphilic Drosophila and their Host Plants in the Sonoran Desert and Its Relation to Temperate and Tropical Associations

Abstract

A survey was made in the Sonoran Desert of yeasts living in the decaying arms of five species of cereus cacti and the four species of Drosophila that utilize them as host plants. The most common yeasts among 132 isolates from the cacti and 187 isolates from the files, respectively, were: Pichia membranaefaciens (45% and 67%), Candida ingens (17% and 4%), Torulopsis sonorensis (12% and 11%), and Cryptococcus cereanus (8% and 7%). Eighty—eight percent of the 66 initial isolates of P. membranaefaciens from Drosophila pachea and its host, senita cactus, assimilated D—xylose while only 12% of the remaining 257 initial isolates did so. Nineteen of the 20 isolates of T. sonorensis from files were found in Drosophila mojavensis and 12 of the 14 isolates of Cryptococcus cereanus came from D. pachea. The highest mean number of yeast species per cactus was 2.77 plus or minus 0.68 in organpipe cactus and per fly was 1.63 plus or minus 0.53 in D. pachea. The flies usually carried fewer yeast species than were found in the host plant but D. pachea had almost the same mean and variance as its host, senita cactus, which had 1.64 @+ 0.40. Yeast species diversity and average niche overlap have the following rank order among habitats and localities: temperate trees > temperate flies > tropical flies > desert cacti > desert flies. Habitat diversity and average niche width show: tropical flies > temperate flies and desert cacti > desert flies and temperate trees. The physiological properties of the desert yeasts are most similar to those of the tropical yeast. However, desert yeasts have similarities with yeasts of temperate trees. Both have low fermentative ability and high assimilation ability of several alcohols and acids. The genus Pichia is by far the most common yeast genus associated with Drosophila in all habitats analyzed (36% of 1,426 isolates).