Abstract
A continual question that concerns researchers using digestion techniques for the study of asbestos load in tissue is, “What percentage of the total fibers remains adhered to the walls of the preparative vials after digestion?“In order to get a perspective on this problem, a test series was established which enabled us to define this residual fiber content following several variations in the rinsing procedure. The test material for all studies was a digested piece of lung tissue from a former asbestos worker. The digestion procedure used was based on the bleach digestion technique presented by Smith and Naylor. This tissue contained 3x108 fibers and 8x105 ferruginous bodies per gram and had a fiber per ferruginous body ratio of 37.5. Aliquots (1.5 ml) of the digest were used to create reproducible standards.
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