Abstract
The genetic variation of wild and hatchery populations of swimming crab Portunus trituberculatus based on observation of amplified fragment length polymorphism (AFLP) was described. A group of 180 genotypes belonging to five wild samples, Dongying (DY), Weifang (WF), Weihai (WH), Qingdao (QD), Rizhao (RZ) and one hatchery population, Yantai (YT) were screened using eight different AFLP primer combinations. A total of 396 loci were screened in the six studied populations. 49.9%, 48.5%, 52.3%, 51.2%, 50.3% and 44.5% of these loci were polymorphic among the individuals tested in the DY, WF, WH, QD, RZ and YT populations, respectively. The number of polymorphic loci detected by single primer combinations ranged from 22 to 37. The average heterozygosity of the DY, WF, WH, QD, RZ and YT populations were 0.087, 0.085, 0.096, 0.092, 0.090 and 0.068, respectively. The WH population showed the highest genetic diversity in terms of total number of AFLP bands, total number of polymorphic bands, average heterozygosity and percentage of low frequency (0-0.2) polymorphic loci among all the populations, while the WF population was the lowest among the wild populations. Compared with the wild populations, the hatchery population showed a low genetic viability.
Highlights
The swimming crab Portunus trituberculatus, is distributed mainly on sandy and muddy bottoms in the coastal waters of Japan, Korea, and China
amplified fragment length polymorphism (AFLP) analysis of 180 swimming crab individuals using eight primer combinations produced a total of 396 scoreable bands, of which 49.9%, 48.5%, 52.3%, 51.2%, 50.3% and 44.5% were polymorphic over all the individuals tested in the Dongying, Weifang, Weihai, Qingdao, Rizhao and Yantai populations, respectively (Table 2)
The percentage of low frequency polymorphic bands in the hatchery population was lower (P
Summary
The swimming crab Portunus trituberculatus, is distributed mainly on sandy and muddy bottoms in the coastal waters of Japan, Korea, and China. It is one of the most common edible crabs in China and Korea and supports a large crab fishery and aquaculture in China [1]. It is being cultured in North China, especially in Shandong Peninsula, because of its high commercial interest. Long-term conservation of genetic diversity is important for any species [5]. Swimming crab resource management and enhancement are a recent practice to maintain long-term resource sustainability. A basic understanding of stock structure among geographical swimming crab samples is required
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