An analysis of differentially expressed and differentially m6A-modified transcripts in soybean roots treated with lead

Abstract

Lead is one of the most common toxic heavy metal pollutants in nature, and exposure to lead can cause serious toxicity to many organisms. In this study, we collected root growth data from soybean plants exposed to lead for seven days and confirmed that lead significantly inhibited root growth. We performed a transcriptome-wide m6A methylation analysis to study the response of soybean RNA methylation groups to lead. The m6A modified regions were enriched near the 3′UTR region and stop codon, and m6A methylation was positively correlated with transcript abundance. In the presence of lead, the transcriptome range of m6A RNA methylation peaks increased, and we identified 1144 m6A modification peaks and 1094 differentially expressed genes. The integration of m6A methylation and transcriptomic results enabled us to identify 16 candidate genes whose transcripts were differentially methylated and differentially expressed under lead stress. Annotation results suggest that these genes may promote abiotic stress tolerance by impacting lead uptake, transport, and accumulation through ROS pathways, enzymes, transporters, and hormones. These results provide candidate genes for future studies of lead stress tolerance mechanisms in soybean roots and provide genetic resources for studying plant heavy metal stress in soybean breeding.