An analysis of bacteria separation and filtration from blood sample using passive methods

Abstract

In the development of the biological device separation of bioparticles plays a vital role in the field of health care and diagnostics. One of the mechanism which satisfies the demands of precision, high throughput, less clogging problem and multiple bioparticle separation is Hydrodynamic Filtration (HDF) Technique uses fluid flow rates, arrays/grooves, streamlines and other related characteristics/parameters by utilizing a Colander/Strainer microchannel network. By using a commercial software COMSOL Multiphysics a proposed analysis and simulation for bioparticle separation through Colander/Strainer geometrical network were studied with a structure of slanted angles 15°, 25°, 35° and 90° in which a bioparticles were introduced via inlet systematically with definite fluid flow rates with addition of buffer. However the larger bioparticles (K. pneumoniae/mammalian cells) and smaller bioparticles (Blood cells) were been formulated (injected) depending on the individual flowrates in a system of microfluidics and its streamlines due to which smaller bioparticles were towed (dragged) with the streamlines directions and larger bioparticles were robust towards the upper sidewalls of a microchannel as per the flow rates as well as other parameters applied and the separation of targeted bioparticles were achieved in a precise manner through a desired outlets. A successful performance for Colander/strained microchannel network was achieved by simulation for separation of targeted bioparticles, specifically a design consisting of 25° and 90° Colander/strained structure provided a precise targeted bioparticle separation through outlet-4 and outlet-1 for larger bioparticles dependent on size and mass and other bioparticles through the different outlets available according to the parameters registered.