An amphotericin B-fluorescein conjugate as a powerful probe for biochemical studies of the membrane.

Abstract

Not only skin deep: A fluorescein-amphotericin B conjugate with a new piperazine linker (see structure) was prepared and used as a probe of the biomembrane and the mechanism of action of amphotericin B both in vivo and in liposomal experiments. The amphotericin B analogue localizes at the membrane of yeast cells but is internalized by mammalian cells.