Abstract
An efficient protocol for high frequency in vitro regeneration of Meizotropis pellita an endangered and endemic plant was developed. In vitro germination of the seeds of Patwa was achieved successfully after incubation for 10 -15 days and plantlets up to a height of 5 -7 cm with profuse rooting were observed after incubation for 7 weeks in hormone free MS medium. This in vitro germination totally reduced contamination that used to occur in the explant taken from the field. Callus induction and proliferation were observed in leaf explants after 15 -20 days of incubation in MS medium containing 2 -4, D (9.06 μM) alone or in combination with 2 -4, D (9.06 μM) + 2-iP (7.38 μM). Shoot regeneration was achieved from 2 months old callus explant in MS medium supplemented with BA (17.6 μM) + GA3 (1.0 μM). Shoot multiplication was also achieved from cotyledonary node of M. pellita in MS medium supplemented with Kinetin + GA3 (4.6 μM + 1.0 μM) or BA (13.2, 17.6 μM) + GA3 (1.0 μM) after 30 -45 days of incubation. IBA (4.9 μM) was more effective in root regeneration from micro shoots. The plantlets after acclimatization over a period of 1 month were further hardened in a polyhouse for two months. On the basis of available literature this is the first and significant study regarding the comparative effect of different PGRs on in-vitro propagation of Meizotropis pellita by using different explants and their subsequent effect on rooting. This significant study could be useful for large scale production of successfully hardened plants and conservation of this shrub.
Highlights
In vitro germination of the seeds of Patwa was achieved successfully after incubation for 10 - 15 days and plantlets up to a height of 5 - 7 cm with profuse rooting were observed after incubation for 7 weeks in hormone free MS medium
There are three major sites in Patwadanger where it is blooming in groups of some plants and at present an estimated 150 - 300 plants of M. pellita are left which too are in the verge of extinction
In vitro germination of seeds of Patwa was obtained successfully after incubation for 15 - 20 days and plantlets up to height of 5 - 7 cm. with profuse rooting were observed after incubation for 7 weeks in hormone free MS medium [Photoplate 1(A)]
Summary
Patwa is an angiosperm belonging to the family Fabaceae (Papilionaceae) This plant is a shrub with stout, woody perennial rootstock from which several erect shoots up to 6 feet high and 0.75 inch diameter are annually produced. There are three major sites in Patwadanger where it is blooming in groups of some plants and at present an estimated 150 - 300 plants of M. pellita are left which too are in the verge of extinction. In nature it is under critical selection because of increased deforestation, habitat fragmentation, forest fires, human interference, ignorance of the people and changing climatic conditions. This species has very small population and grows in very specialized and sensitive habitats; any further change and ecological disturbance are bound to cause their total extinction from this region [1]
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