Abstract

AbstractA new laboratory‐based method for obtaining gram quantities of a principal heat‐unstable thaumatin‐like protein (VVTL1), in wine is presented. Separation and purification from grape juice was achieved by cation exchange and ultra‐filtration. Purity was confirmed by HPLC, sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE), gel permeation chromatography (GPC) and high‐sensitivity amino acid analysis. This alternative method is more rapid and compact and can efficiently produce much larger quantities (>100×) than the current laboratory technique. Copyright © 2007 Curtin University of Technology and John Wiley & Sons, Ltd.

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