Abstract
ABSTRACT: A novel cost-effective in vitro regeneration protocol has been evolved for the therapeutically important Ocimum citriodorum Vis. In the present study, table sugar (3%) and isabgol (Psyllium husk) (3.5%) were used as an alternate source of carbon and gelling agent respectively in Murashige and Skoog’s (MS) medium. The explant used in the current study was nodal segment. A significant observation revealed that all the cultures resulted in shoot induction and maximum number of shoots/ culture (6.04) and their average length (2.15 cm) was obtained on modified MS-medium supplemented with table sugar, isabgol and BAP. However, best root induction (95.83%) was obtained on ½ MS-medium augmented with table sugar (3%) , isabgol (3.5%) and NAA. An increase in average number of roots per shoot (6.91%) as well as average root length (2.73 cm) was also observed in the same modified medium. The in vitro regenerated plantlets were successfully transferred to the field and no notable variation was observed in their morphology. The overall cost of culture medium for in vitro propagation of O. citriodorum Vis. was reduced significantly by 92.69% when agar and sucrose were replaced by isabgol and table sugar, respectively.
Highlights
A novel cost-effective in vitro regeneration protocol has been evolved for the therapeutically important Ocimum citriodorum Vis
The overall cost of culture medium for in vitro propagation of O. citriodorum Vis. was reduced significantly by 92.69% when agar and sucrose were replaced by isabgol and table sugar, respectively
The present study reports, successful costcutting of Murashige and Skoog (MS) medium by substituting agar and sucrose with isabgol and table sugar, respectively for micropropagation of medicinally important O. citriodorum Vis
Summary
A novel cost-effective in vitro regeneration protocol has been evolved for the therapeutically important Ocimum citriodorum Vis. In the present study, table sugar (3%) and isabgol (Psyllium husk) (3.5%) were used as an alternate source of carbon and gelling agent respectively in Murashige and Skoog’s (MS) medium. The overall cost of culture medium for in vitro propagation of O. citriodorum Vis. was reduced significantly by 92.69% when agar and sucrose were replaced by isabgol and table sugar, respectively. (Lemon basil) is an important medicinal plant, abundant in volatile aromatic essential oils and is known to possess culinary properties1,2 It is an interspecific hybrid obtained between Ocimum basilicum and O. americanum. The present study reports, successful costcutting of MS medium by substituting agar and sucrose with isabgol and table sugar, respectively for micropropagation of medicinally important O. citriodorum Vis. has been standardized in the present study. Nodal segments inoculated on MS-medium having sucrose (3%) + agar (0.7%) + BAP (0.5 mg/l) served as control
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