Abstract
An α-1, 3-glucanase was detected in the culture supernatant of a micro-organism, which was isolated from soil on agar medium containing α-1, 3-glucan as sole carbon source. The isolated strain was characterized as a strain of Streptomyces, tentatively named KI-8. This enzyme required α-1, 3-glucosidic linkage as an inducer. The optimum conditions for enzyme production were studied. The enzyme was purified by (NH4)2SO4 precipitation, column chromatography on DEAE-cellulose and P (phospho)-cellulose. To eliminate the concomitant β-1, 3-glucanase activity, partially purified enzyme preparation was passed through a column packed with pachyman. Final purification was accomplished by the adsorption chromatography using Sephadex G-150 from which the α-1, 3-glucanase was eluted with a solution of α-1, 3-linked gluco-oligo-saccharides. The purified enzyme was electrophoretically homogeneous and had a molecular weight of approximately 78, 000 by SDS-polyacrylamide gel electrophoresis.
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