Abstract
Oxyfunctionalisation reactions in neat substrate still pose a challenge for biocatalysis. Here, we report an alginate-confined peroxygenase-CLEA to catalyse the enantioselective epoxidation of cis-β-methylstyrene in a solvent-free reaction system achieving turnover numbers of 96 000 for the biocatalyst and epoxide concentrations of 48 mM.
Highlights
Oxyfunctionalisation reactions in neat substrate still pose a challenge for biocatalysis
We report an alginate-confined peroxygenase-CLEA to catalyse the enantioselective epoxidation of cis-b-methylstyrene in a solvent-free reaction system achieving turnover numbers of 96 000 for the biocatalyst and epoxide concentrations of 48 mM
Increasing the starting material concentration is of utmost importance to increase the economic viability and environmental friendliness of such biocatalytic reactions.[4]
Summary
We report an alginate-confined peroxygenase-CLEA to catalyse the enantioselective epoxidation of cis-b-methylstyrene in a solvent-free reaction system achieving turnover numbers of 96 000 for the biocatalyst and epoxide concentrations of 48 mM. (b) Epoxidation of cis-b-methylstyrene by immobilised rAaeUPO with tert-butyl hydroperoxide (tBuOOH) as oxidant. We set out to immobilise a peroxygenase in an alginate matrix and evaluate its catalytic activity under non aqueous reaction conditions.
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