Abstract
Polyelectrolyte PTPEPyH , containing tetraphenylethene (TPE) and pyridinium, was synthesized. Its optical properties were investigated with spectroscopies and the results showed that there were two emission-enhanced stages in the interaction between positive-charged PTPEPyH and negative-charged biomacromolecule heparin. The mechanism was very different due to the reduced non-radiative energy loss and the change of surroundings. The polyelectrolyte PTPEPyH , compared with ChS and HA, also showed high selectivity for heparin in the buffer solution and could be used as a potential bio-probe for heparin quantification in the clinical full range.
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