Abstract
A ratiometric fluorescence detection method for trace amount of uranyl ion (UO22+) was developed in this study, based on aggregation induced emission enhancement (AIEE) characteristics of 3-hydroxyflavone salicylaldehyde azine (HFSA) and luminescent signal of UO22+-HFSA complex in soluble state. The fluorescent ratio (I457/I534) was linearly related to the concentration of UO22+ in the range of 0.5–20 ppb (part per billion). Analytical data showed that a detection limit of 0.5 ppb was achieved with the relative standard deviation (R.S.D.) 1.3% (n=5). The proposed method could also be utilized for imaging UO22+ in living cells by ratiometric fluorescence changes.
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