Abstract
We have developed an inexpensive portable microarray reader that can be applied to standard microscope slide-based arrays and other array formats printed on chemically modified surfaces. Measuring only 19 cm in length, the imaging device is portable and may be applicable to both triage and clinical settings. For multiplexing and adaptability purposes, it can be modified to work with multiple excitation/emission wavelengths. Our device is shown to be comparable to a commercial laser scanner when detecting both streptavidin-biotin and antibody interactions. This paper presents the development and characterization of a handheld microarray imager and directly compares it with a commercial scanner.
Highlights
Antibody-based immunoassays are currently the most commonly used tools to measure biomarkers in patient samples [1,2]
In this paper we describe a new alternative to microarray scanners that would meet the needs of the market
While the handheld microarray reader described here lacks some of the features and functions of the larger laser scanners, its small and minimalistic design makes it ideal for specialized uses such as rapid diagnosis in medical clinics and other POC settings
Summary
Antibody-based immunoassays are currently the most commonly used tools to measure biomarkers in patient samples [1,2] Since their first use in the 1950s, newer and more efficient antibodies and other affinity reagents have been created to improve assay sensitivity. That utilizes a small laser diode, but their use of a film-based camera precluded rapid analysis of the array Another group later demonstrated a digital portable microarray reader [15] using an ultra-bright. While the handheld microarray reader described here lacks some of the features and functions of the larger laser scanners, its small and minimalistic design makes it ideal for specialized uses such as rapid diagnosis in medical clinics and other POC settings. In recognition that accuracy is of the utmost importance for acceptance by medical providers, we provide here a direct comparison of our handheld imager to a commercial scanner
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