Abstract
A method is described for the construction of a highly sensitive electrochemical biosensor for the detection of acrylamide, based on covalent immobilization of hemoglobin (Hb) onto carboxylated multiwalled carbon nanotube/copper nanoparticle/polyaniline (c-MWCNT/CuNP/PANI) composite electrodeposited onto pencil graphite (PG) electrode. The enzyme electrode was characterized by cyclic voltammetry (CV), scanning electron microscopy (SEM), X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, and electrochemical impedance spectroscopy (EIS). The biosensor showed an optimal response at pH5.5 (0.1M sodium acetate buffer) and 35°C when operated at 20mVs−1. The biosensor exhibited low detection limit (0.2nM) with high sensitivity (72.5μA/nM/cm2), fast response time (<2s), and wide linear range (5nM to 75mM). Analytical recovery of added acrylamide was 95.40 to 97.56%. Within- and between-batch coefficients of variation were 2.35 and 4.50%, respectively. The enzyme electrode was used 120 times over a period of 100days, when stored at 4°C.
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