Abstract
Azithromycin has become one of the most widely prescribed macrolide antibiotics due to the pharmacokinetic profile and broad spectrum of activity. A method for determining the level of azithromycin in ferret feces involving drying, methylene chloride extraction, solid phase extraction (SPE) clean-up, and HPLC analysis was developed. The method was linear from 0.8 to 3.2 mg/mL with a mean recovery of 97% (0.6% RSD for 50 mg spiked simulated feces). The analysis was developed to quantitate approximately 50 mg of azithromycin in approximately 0.6g of dried feces. The HPLC method uses a pH 7.5 phosphate buffered mobile phase with a conventional C18 column and UV detection at 205 nm. The HPLC system is robust and column life is at least 1 month (∼ 200 injections).
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