An accurate dihydrouracil/uracil determination using improved high performance liquid chromatography method for preventing fluoropyrimidines-related toxicity in clinical practice

Abstract

An accurate and improved HPLC method was set up to measure both dihydrouracil (UH 2) and uracil (U) in plasma, and to assess their ratio. Analytes retention time, separation and peak purity were greatly optimized with a Hypercarb column and a diode array detector. U and UH 2 limits of quantification were 1.25 and 0.625 ng/mL. U and UH 2 within-day precisions were 0.9–2.3% and 0.7–5.6%. Between-day precisions were 1.3–5.3% and 1.3–7.1%. Accuracy was 0.1–6.1%. UH 2/U ratio between-day variability was low, but ratio decreased from 02:00 p.m. This method is now used in practice to detect patients at risk of fluoropyrimidine toxicity and to individually adapt the dosage.